Knockdown of pyruvate kinase M2 suppresses bladder cancer progression

Abstract Objectives Bladder cancer (BCa) is one of the most frequently diagnosed cancers urinary tract and has a high mortality. The M2 splice isoform pyruvate kinase (PKM2) key regulator Warburg effect in cells. This study aimed to evaluate metabolic alterations biological behaviours after knocking down PKM2. Methods In this study, 36 pairs BCa tissues adjacent normal were collected analyse expression level PKM2 explore relationship between tumour patient status. After knockdown T24 cells, cell survival, migration, invasion, glucose uptake, lactate production, apoptosis detected. tumour-forming ability PKM2-reducing cells was examined vivo . Results results showed that correlates with stage grade. decreases consumption production suppresses proliferation, invasion while increasing reactive oxygen species levels vitro nude mouse models, reduced xenograft orthotopic size. Moreover, decreased vimentin fibronectin increased E-cadherin expression. Analysis high-throughput sequencing data revealed may also be associated processes diseases. Conclusions Overall, these indicate therapeutic target for patients.